Identification of an epitope of SARS-coronavirus nucleocapsid protein.
Identifieur interne : 005F76 ( Main/Exploration ); précédent : 005F75; suivant : 005F77Identification of an epitope of SARS-coronavirus nucleocapsid protein.
Auteurs : Ying Lin [République populaire de Chine] ; Xu Shen ; Rui Fu Yang ; Yi Xue Li ; Yong Yong Ji ; You Yu He ; Mu De Shi ; Wei Lu ; Tie Liu Shi ; Jin Wang ; Hong Xia Wang ; Hua Liang Jiang ; Jian Hua Shen ; You Hua Xie ; Yuan Wang ; Gang Pei ; Bei Fen Shen ; Jia Rui Wu ; Bing SunSource :
- Cell research [ 1001-0602 ] ; 2003.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Données de séquences moléculaires, Fragments peptidiques (), Fragments peptidiques (immunologie), Humains, Immunisation, Immunoglobuline G (immunologie), Immunoglobuline G (sang), Lapins, Liaison aux protéines (immunologie), Protéines nucléocapside (), Protéines nucléocapside (immunologie), Protéines nucléocapside (isolement et purification), Spécificité des anticorps (immunologie), Séquence d'acides aminés, Technique de Western, Test ELISA, Vecteurs génétiques (génétique), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (immunologie), Épitopes (), Épitopes (immunologie), Épitopes (isolement et purification).
- MESH :
- génétique : Vecteurs génétiques, Virus du SRAS.
- immunologie : Fragments peptidiques, Immunoglobuline G, Liaison aux protéines, Protéines nucléocapside, Spécificité des anticorps, Virus du SRAS, Épitopes.
- isolement et purification : Protéines nucléocapside, Épitopes.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Animaux, Données de séquences moléculaires, Fragments peptidiques, Humains, Immunisation, Lapins, Protéines nucléocapside, Séquence d'acides aminés, Technique de Western, Test ELISA, Virus du SRAS, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Antibody Specificity (immunology), Blotting, Western, Enzyme-Linked Immunosorbent Assay, Epitopes (chemistry), Epitopes (immunology), Epitopes (isolation & purification), Genetic Vectors (genetics), Humans, Immunization, Immunoglobulin G (blood), Immunoglobulin G (immunology), Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (isolation & purification), Peptide Fragments (chemistry), Peptide Fragments (immunology), Protein Binding (immunology), Rabbits, SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , chemistry : Epitopes, Nucleocapsid Proteins, Peptide Fragments.
- chemistry : SARS Virus.
- genetics : Genetic Vectors, SARS Virus.
- immunology : Antibody Specificity, Epitopes, Immunoglobulin G, Nucleocapsid Proteins, Peptide Fragments, Protein Binding, SARS Virus.
- chemical , isolation & purification : Epitopes, Nucleocapsid Proteins.
- Amino Acid Sequence, Animals, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Humans, Immunization, Molecular Sequence Data, Rabbits.
Abstract
The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, it was confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be usefull for the study of SARS-CoV.
DOI: 10.1038/sj.cr.7290158
PubMed: 12862314
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003280
- to stream PubMed, to step Curation: 003280
- to stream PubMed, to step Checkpoint: 003220
- to stream Ncbi, to step Merge: 000227
- to stream Ncbi, to step Curation: 000227
- to stream Ncbi, to step Checkpoint: 000227
- to stream Main, to step Merge: 006467
- to stream Main, to step Curation: 005F76
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of an epitope of SARS-coronavirus nucleocapsid protein.</title><author><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea><wicri:noRegion>Shanghai 200031</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Xu" sort="Shen, Xu" uniqKey="Shen X" first="Xu" last="Shen">Xu Shen</name></author><author><name sortKey="Yang, Rui Fu" sort="Yang, Rui Fu" uniqKey="Yang R" first="Rui Fu" last="Yang">Rui Fu Yang</name></author><author><name sortKey="Li, Yi Xue" sort="Li, Yi Xue" uniqKey="Li Y" first="Yi Xue" last="Li">Yi Xue Li</name></author><author><name sortKey="Ji, Yong Yong" sort="Ji, Yong Yong" uniqKey="Ji Y" first="Yong Yong" last="Ji">Yong Yong Ji</name></author><author><name sortKey="He, You Yu" sort="He, You Yu" uniqKey="He Y" first="You Yu" last="He">You Yu He</name></author><author><name sortKey="Shi, Mu De" sort="Shi, Mu De" uniqKey="Shi M" first="Mu De" last="Shi">Mu De Shi</name></author><author><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name></author><author><name sortKey="Shi, Tie Liu" sort="Shi, Tie Liu" uniqKey="Shi T" first="Tie Liu" last="Shi">Tie Liu Shi</name></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name></author><author><name sortKey="Wang, Hong Xia" sort="Wang, Hong Xia" uniqKey="Wang H" first="Hong Xia" last="Wang">Hong Xia Wang</name></author><author><name sortKey="Jiang, Hua Liang" sort="Jiang, Hua Liang" uniqKey="Jiang H" first="Hua Liang" last="Jiang">Hua Liang Jiang</name></author><author><name sortKey="Shen, Jian Hua" sort="Shen, Jian Hua" uniqKey="Shen J" first="Jian Hua" last="Shen">Jian Hua Shen</name></author><author><name sortKey="Xie, You Hua" sort="Xie, You Hua" uniqKey="Xie Y" first="You Hua" last="Xie">You Hua Xie</name></author><author><name sortKey="Wang, Yuan" sort="Wang, Yuan" uniqKey="Wang Y" first="Yuan" last="Wang">Yuan Wang</name></author><author><name sortKey="Pei, Gang" sort="Pei, Gang" uniqKey="Pei G" first="Gang" last="Pei">Gang Pei</name></author><author><name sortKey="Shen, Bei Fen" sort="Shen, Bei Fen" uniqKey="Shen B" first="Bei Fen" last="Shen">Bei Fen Shen</name></author><author><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia Rui" last="Wu">Jia Rui Wu</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12862314</idno><idno type="pmid">12862314</idno><idno type="doi">10.1038/sj.cr.7290158</idno><idno type="wicri:Area/PubMed/Corpus">003280</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003280</idno><idno type="wicri:Area/PubMed/Curation">003280</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003280</idno><idno type="wicri:Area/PubMed/Checkpoint">003220</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003220</idno><idno type="wicri:Area/Ncbi/Merge">000227</idno><idno type="wicri:Area/Ncbi/Curation">000227</idno><idno type="wicri:Area/Ncbi/Checkpoint">000227</idno><idno type="wicri:doubleKey">1001-0602:2003:Lin Y:identification:of:an</idno><idno type="wicri:Area/Main/Merge">006467</idno><idno type="wicri:Area/Main/Curation">005F76</idno><idno type="wicri:Area/Main/Exploration">005F76</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of an epitope of SARS-coronavirus nucleocapsid protein.</title><author><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea><wicri:noRegion>Shanghai 200031</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Xu" sort="Shen, Xu" uniqKey="Shen X" first="Xu" last="Shen">Xu Shen</name></author><author><name sortKey="Yang, Rui Fu" sort="Yang, Rui Fu" uniqKey="Yang R" first="Rui Fu" last="Yang">Rui Fu Yang</name></author><author><name sortKey="Li, Yi Xue" sort="Li, Yi Xue" uniqKey="Li Y" first="Yi Xue" last="Li">Yi Xue Li</name></author><author><name sortKey="Ji, Yong Yong" sort="Ji, Yong Yong" uniqKey="Ji Y" first="Yong Yong" last="Ji">Yong Yong Ji</name></author><author><name sortKey="He, You Yu" sort="He, You Yu" uniqKey="He Y" first="You Yu" last="He">You Yu He</name></author><author><name sortKey="Shi, Mu De" sort="Shi, Mu De" uniqKey="Shi M" first="Mu De" last="Shi">Mu De Shi</name></author><author><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name></author><author><name sortKey="Shi, Tie Liu" sort="Shi, Tie Liu" uniqKey="Shi T" first="Tie Liu" last="Shi">Tie Liu Shi</name></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name></author><author><name sortKey="Wang, Hong Xia" sort="Wang, Hong Xia" uniqKey="Wang H" first="Hong Xia" last="Wang">Hong Xia Wang</name></author><author><name sortKey="Jiang, Hua Liang" sort="Jiang, Hua Liang" uniqKey="Jiang H" first="Hua Liang" last="Jiang">Hua Liang Jiang</name></author><author><name sortKey="Shen, Jian Hua" sort="Shen, Jian Hua" uniqKey="Shen J" first="Jian Hua" last="Shen">Jian Hua Shen</name></author><author><name sortKey="Xie, You Hua" sort="Xie, You Hua" uniqKey="Xie Y" first="You Hua" last="Xie">You Hua Xie</name></author><author><name sortKey="Wang, Yuan" sort="Wang, Yuan" uniqKey="Wang Y" first="Yuan" last="Wang">Yuan Wang</name></author><author><name sortKey="Pei, Gang" sort="Pei, Gang" uniqKey="Pei G" first="Gang" last="Pei">Gang Pei</name></author><author><name sortKey="Shen, Bei Fen" sort="Shen, Bei Fen" uniqKey="Shen B" first="Bei Fen" last="Shen">Bei Fen Shen</name></author><author><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia Rui" last="Wu">Jia Rui Wu</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author></analytic><series><title level="j">Cell research</title><idno type="ISSN">1001-0602</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Antibody Specificity (immunology)</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes (chemistry)</term><term>Epitopes (immunology)</term><term>Epitopes (isolation & purification)</term><term>Genetic Vectors (genetics)</term><term>Humans</term><term>Immunization</term><term>Immunoglobulin G (blood)</term><term>Immunoglobulin G (immunology)</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (chemistry)</term><term>Nucleocapsid Proteins (immunology)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>Peptide Fragments (chemistry)</term><term>Peptide Fragments (immunology)</term><term>Protein Binding (immunology)</term><term>Rabbits</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Données de séquences moléculaires</term><term>Fragments peptidiques ()</term><term>Fragments peptidiques (immunologie)</term><term>Humains</term><term>Immunisation</term><term>Immunoglobuline G (immunologie)</term><term>Immunoglobuline G (sang)</term><term>Lapins</term><term>Liaison aux protéines (immunologie)</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines nucléocapside (isolement et purification)</term><term>Spécificité des anticorps (immunologie)</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Test ELISA</term><term>Vecteurs génétiques (génétique)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Épitopes ()</term><term>Épitopes (immunologie)</term><term>Épitopes (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Epitopes</term><term>Nucleocapsid Proteins</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genetic Vectors</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Vecteurs génétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Fragments peptidiques</term><term>Immunoglobuline G</term><term>Liaison aux protéines</term><term>Protéines nucléocapside</term><term>Spécificité des anticorps</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Specificity</term><term>Epitopes</term><term>Immunoglobulin G</term><term>Nucleocapsid Proteins</term><term>Peptide Fragments</term><term>Protein Binding</term><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Epitopes</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Immunization</term><term>Molecular Sequence Data</term><term>Rabbits</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Fragments peptidiques</term><term>Humains</term><term>Immunisation</term><term>Lapins</term><term>Protéines nucléocapside</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Test ELISA</term><term>Virus du SRAS</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, it was confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be usefull for the study of SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="He, You Yu" sort="He, You Yu" uniqKey="He Y" first="You Yu" last="He">You Yu He</name><name sortKey="Ji, Yong Yong" sort="Ji, Yong Yong" uniqKey="Ji Y" first="Yong Yong" last="Ji">Yong Yong Ji</name><name sortKey="Jiang, Hua Liang" sort="Jiang, Hua Liang" uniqKey="Jiang H" first="Hua Liang" last="Jiang">Hua Liang Jiang</name><name sortKey="Li, Yi Xue" sort="Li, Yi Xue" uniqKey="Li Y" first="Yi Xue" last="Li">Yi Xue Li</name><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name><name sortKey="Pei, Gang" sort="Pei, Gang" uniqKey="Pei G" first="Gang" last="Pei">Gang Pei</name><name sortKey="Shen, Bei Fen" sort="Shen, Bei Fen" uniqKey="Shen B" first="Bei Fen" last="Shen">Bei Fen Shen</name><name sortKey="Shen, Jian Hua" sort="Shen, Jian Hua" uniqKey="Shen J" first="Jian Hua" last="Shen">Jian Hua Shen</name><name sortKey="Shen, Xu" sort="Shen, Xu" uniqKey="Shen X" first="Xu" last="Shen">Xu Shen</name><name sortKey="Shi, Mu De" sort="Shi, Mu De" uniqKey="Shi M" first="Mu De" last="Shi">Mu De Shi</name><name sortKey="Shi, Tie Liu" sort="Shi, Tie Liu" uniqKey="Shi T" first="Tie Liu" last="Shi">Tie Liu Shi</name><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name><name sortKey="Wang, Hong Xia" sort="Wang, Hong Xia" uniqKey="Wang H" first="Hong Xia" last="Wang">Hong Xia Wang</name><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><name sortKey="Wang, Yuan" sort="Wang, Yuan" uniqKey="Wang Y" first="Yuan" last="Wang">Yuan Wang</name><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia Rui" last="Wu">Jia Rui Wu</name><name sortKey="Xie, You Hua" sort="Xie, You Hua" uniqKey="Xie Y" first="You Hua" last="Xie">You Hua Xie</name><name sortKey="Yang, Rui Fu" sort="Yang, Rui Fu" uniqKey="Yang R" first="Rui Fu" last="Yang">Rui Fu Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005F76 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005F76 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12862314
|texte= Identification of an epitope of SARS-coronavirus nucleocapsid protein.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12862314" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |